| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
| IP Immunoprecipitation Read more |
| ChIP-qPCR 定量のPCRとクロマチン免疫沈降（ChIP）が相まり、既知のゲノム結合部位でのタンパク質-DNA相互作用を調べる事に利用できます。ゲノム結合部位が不明の場合は、プロモーターのような潜在的な制御領域に対してqPCRプライマーを設計することもできます。ChIP-qPCRは、リアルタイムPCRを実行するコストが最小であるため、異なる実験条件にわたって特定の遺伝子および潜在的な制御領域に焦点を当てた研究においてとても有利です。また、この技術は現在、細胞分化、腫瘍抑制遺伝子のサイレンシング、およ... Read more |
HA tag monoclonal antibody (sample size)
Monoclonal antibody raised in mouse against the HA tag (amino acid sequence YPYDVPDYA) using a KLH-conjugated synthetic peptide. The HA antibody recognizes the influenza Hemagglutinin epitope which is extensively used as a protein tag in expression vectors. This antibody is extremely specific and allows unambiguous identification and quantitative analysis of HA tagged proteins.
| Lot | 003 |
|---|---|
| Concentration | 1.0 µg/µl |
| Species reactivity | Specific for HA-tagged fusion proteins expressed in bacteria, insect, mammalian cells |
| Type | Monoclonal |
| Purity | Protein A purified |
| Host | Mouse |
| Storage Conditions | Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles. |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution * | References |
|---|---|---|
| Western Blotting | 1:1,000 - 1:3,000 | Fig 1 |
| IP/ChIP | 1:150 - 1:200 | |
| IS | 1:500 - 1:1,000 |
* Please note that optimal antibody amount should be determined by the end-user for each specific assay condition.
- Validation Data
Figure 1. Western blot analysis using the Diagenode monoclonal antibody against the HA protein tag
Western blot was performed on whole cell extracts from HEK293 cells transfected with an HA-tagged expression vector using the Diagenode monoclonal antibody directed against HA (Cat. No. MAb-190-050). The antibody was used at a dilution of 1:1,000. Figure 1 shows the results for untransfected cells, used as a negative control (lane 1) and for cells transfected with a HA-tagged protein (lane 2). The MW marker (in kDa) is shown on the left. A single band is clearly visible in lane 2, but absent in lane 1 showing the high specificity of the HA antibody. - 出版物
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