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<p><strong>Figure 1. Sequencing profiles of µChIPmentation libraries </strong><br />Chromatin preparation and immunoprecipitation have been performed on 10.000 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 SI for Tagmented libraries (Cat. No. C010111032) using K562 cells. The Diagenode antibodies targeting H3K4me3 (Cat. No. C15410003), H3K27ac (Cat. No. C15410196), H3K27me3 (Cat. No. C15410195) and H3K9me3 (Cat. No. C15410193) have been used.</p>
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<p><strong>Figure 1. Sequencing profiles of µChIPmentation libraries </strong><br />Chromatin preparation and immunoprecipitation have been performed on 10.000 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 SI for Tagmented libraries (Cat. No. C010111032) using K562 cells. The Diagenode antibodies targeting H3K4me3 (Cat. No. C15410003), H3K27ac (Cat. No. C15410196), H3K27me3 (Cat. No. C15410195) and H3K9me3 (Cat. No. C15410193) have been used.</p>
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<p>The <strong>24 SI for tagmented libraries</strong> includes 24 single primer indexes for multiplexing up to 24 samples. The primer indexes of the 24 SI Tagmented libraries are compatible with any <strong>tagmentation-based library preparation protocols</strong>, such as <a href="https://www.diagenode.com/en/categories/chromatin-ip-chipmentation">ChIPmentation</a>, <a href="https://www.diagenode.com/en/categories/atac-seq">ATAC-seq</a> or <a href="https://www.diagenode.com/en/categories/cutandtag">CUT&Tag</a> technologies.</p>
<p>Additionally, the Unique Dual indexes for tagmented libraries are available. <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">Read more</a></p>',
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<p><strong>Figure 1. Sequencing profiles of µChIPmentation libraries </strong><br />Chromatin preparation and immunoprecipitation have been performed on 10.000 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 SI for Tagmented libraries (Cat. No. C010111032) using K562 cells. The Diagenode antibodies targeting H3K4me3 (Cat. No. C15410003), H3K27ac (Cat. No. C15410196), H3K27me3 (Cat. No. C15410195) and H3K9me3 (Cat. No. C15410193) have been used.</p>
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<div class="small-12 medium-8 large-8 columns">
<div id="video"></div>
<h2>ChIPmentation for high-quality and fast <br />ChIP-seq library preparation</h2>
<p>Diagenode’s ChIPmentation technology, based on tagmentation, enables the integration of library preparation during ChIP itself using transposase and sequencing-compatible adaptors. Unlike standard library preparation techniques that require multi-step ligation, ChIPmentation incorporates an easier and shorter protocol.</p>
<p>Different protocols have been optimized bringing the solution for different needs:</p>
<ul class="no-bullet">
<li>✓ <strong>Low cell number</strong> – The kit <a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">µChIPmentation for Histones</a> enables to use as little as 10.000 cells per reaction New!</li>
<li>✓ <strong>Standard cell number</strong> – <a href="https://www.diagenode.com/en/p/chipmentation-kit-for-histones">ChIPmentation kit for Histones</a> - two complete protocols have been optimized: a manual procedure and an automated protocol on the IP-Star Compact Automated System.</li>
<li>✓<strong> Module for library prep using</strong> <strong>tagmentation</strong> – <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">Tag Kit for ChIPmentation</a> can be used with any ChIP protocol</li>
</ul>
<div class="extra-spaced">
<div id="video" align="center"><video width="400" height="250" autoplay="autoplay" muted="" loop="loop" controls="controls" src="https://www.diagenode.com/videos/chipmentation-dgo.mp4" frameborder="0" allowfullscreen="allowfullscreen"></video></div>
<div align="center"><a href="https://www.diagenode.com/pages/form-chipmentation" class="center alert radius button"><i class="fa fa-info"></i> Contact us</a></div>
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<div class="extra-spaced">
<h2>Benefits of the ChIPmentation system for optimal ChIP-seq</h2>
<ul class="nobullet">
<li><i class="fa fa-arrow-circle-right"></i> Ensure high quality sequencing data</li>
<li><i class="fa fa-arrow-circle-right"></i> Perform ChIP-seq on low input samples</li>
<li><i class="fa fa-arrow-circle-right"></i> Enjoy an easy and fast protocol</li>
<li><i class="fa fa-arrow-circle-right"></i> Benefit from the elimination of sequencing adaptor dimers</li>
<li><i class="fa fa-arrow-circle-right"></i> Automate on the IP-Star® to support standardization and save time</li>
</ul>
</div>
<div class="extra-spaced">
<ul class="accordion" data-accordion="" style="margin-left: 0; padding-left: 0;">
<li class="accordion-navigation"><a href="#more" style="color: #13b29c; background-color: transparent; display: inline; padding: 0; margin-left: 0;">ChIPmentation workflow - Read more</a>
<div id="more" class="content"><center><img src="https://www.diagenode.com/img/product/kits/chipmentation-for-histones-workflow.png" alt="Diagenode-ChIPmentation" caption="false" width="728" height="1024" /></center>
<div class="spaced">In traditional ChIP-seq library preparation, the ligation of the adaptors is performed after chromatin immunoprecipitation, in three steps. In the Diagenode ChIPmentation workflow, not only are the adaptors directly incorporated during ChIP, but also the main steps are automated on the Diagenode IP-Star®. The combination of direct adaptor incorporation and automation allows for higher sensitivity from low cell numbers and reproducibility of results.</div>
</div>
</li>
</ul>
</div>
<div class="extra-spaced">
<h2>Complete kits:</h2>
<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#v1" style="color: #13b29c;"><i class="fa fa-caret-right"></i> µChIPmentation for Histones</a>
<div id="v1" class="content">The Diagenode µChIPmentation Kit for Histones is optimized to perform ChIP-seq on as little as 10.000 cells from cell fixation to purified libraries.<br /><a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v2" style="color: #13b29c;"><i class="fa fa-caret-right"></i> ChIPmentation Kit for Histones </a>
<div id="v2" class="content">ChIPmentation is a ligation-free, tagmentation-based protocol that incorporates chromatin immunoprecipitation with NGS library preparation.<br /><a href="https://www.diagenode.com/en/p/chipmentation-kit-for-histones">Read more</a></div>
</li>
</ul>
<h2>Modules:</h2>
<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#v3" style="color: #13b29c;"><i class="fa fa-caret-right"></i> TAG Kit for ChIPmentation</a>
<div id="v3" class="content">The TAG Kit for ChIPmentation has been developed for researchers willing to perform ChIPmentation using their own validated ChIP protocol.<br /><a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">Read more</a></div>
</li>
</ul>
<h2>Primer indexes for tagmented libraries:</h2>
<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#v4" style="color: #13b29c;"><i class="fa fa-caret-right"></i> 24 SI for ChIPmentation</a>
<div id="v4" class="content">The 24 SI for ChIPmentation includes 24 single indexes compliting the TAG Kit for ChIPmentation.<br /><a href="https://www.diagenode.com/en/p/24-si-for-chipmentation">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v5" style="color: #13b29c;"><i class="fa fa-caret-right"></i> 24 SI for Tagmented libraries</a>
<div id="v5" class="content">The 24 SI for tagmented libraries includes 24 single primer indexes for multiplexing up to 24 samples. This set of indexes is compatible with any tagmentation-based library preparation protocols, such as ChIPmentation, ATAC-seq or CUT&Tag technologies.<br /><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v6" style="color: #13b29c;"><i class="fa fa-caret-right"></i> 24 UDI for Tagmented libraries - Set I</a>
<div id="v6" class="content">The 24 UDI (Unique dual indexes) for tagmented libraries – Set I provides combinations of barcodes where each barcode is uniquely attributed to one sample.<br /><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v7" style="color: #13b29c;"><i class="fa fa-caret-right"></i> 24 UDI for Tagmented libraries - Set II</a>
<div id="v7" class="content">The 24 UDI (Unique dual indexes) for tagmented libraries – Set II includes 24 primer pairs for unique dual-indexing and is designed to be combined with the 24 UDI for tagmented libraries – Set I, allowing multiplexing of up to 48 samples for sequencing on Illumina platforms.<br /><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v8" style="color: #13b29c;"><i class="fa fa-caret-right"></i> 24 UDI for Tagmented libraries - Set III</a>
<div id="v8" class="content">The 24 UDI (Unique dual indexes) for tagmented libraries – Set III includes 24 primer pairs for unique dual-indexing. It is designed to be used in combination with other sets of UDI (Set I, II and IV), allowing multiplexing of up to 96 samples for sequencing on Illumina platforms. <br /><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3">Read more</a></div>
</li>
<li class="accordion-navigation"><br />
<div id="v9" class="content">The 24 UDI (Unique dual indexes) for tagmented libraries – Set IV includes 24 primer pairs for unique dual-indexing. It is designed to be used in combination with other sets of UDI (Set I, II and III), allowing multiplexing of up to 96 samples for sequencing on Illumina platforms. <br /><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set4">Read more</a></div>
</li>
</ul>
<h2>Separately available products:</h2>
<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#v10" style="color: #13b29c;"><i class="fa fa-caret-right"></i> Tagmentase (Tn5 transposase) - loaded</a>
<div id="v10" class="content">Diagenode Tagmentase – loaded is a hyperactive Tn5 transposase preloaded with Illumina-compatible sequencing adapters.<br /><a href="https://www.diagenode.com/en/p/tagmentase-loaded-30">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v11" style="color: #13b29c;"><i class="fa fa-caret-right"></i> Tagmentase (Tn5 transposase) - unloaded</a>
<div id="v11" class="content">Diagenode Tagmentase is a hyperactive Tn5 transposase with the ability to cut DNA and insert sequences of interest into any target DNA in one step. This enzyme is not loaded with DNA oligos.<br /><a href="https://www.diagenode.com/en/p/tagmentase">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v12" style="color: #13b29c;"><i class="fa fa-caret-right"></i> Tagmentase Dilution Buffer</a>
<div id="v12" class="content">Diagenode Tagmentase Dilution Buffer is recommended for dilution of Tagmentase (Cat. No. C01070010) before or after transposome assembly.<br /><a href="https://www.diagenode.com/en/p/tagmentase-dilution-buffer">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v13" style="color: #13b29c;"><i class="fa fa-caret-right"></i> Tagmentation Buffer (1x)</a>
<div id="v13" class="content">Diagenode Tagmentation Buffer (1x) is the recommended reagent to perform any tagmentation reactions.<br /><a href="https://www.diagenode.com/en/p/tagmentation-buffer-1x-1ml">Read more</a></div>
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<h3>Features:</h3>
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<li>Compatibility: <strong>tagmentation-based library</strong> preparation protocols</li>
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<p>Get the manual of <a href="https://www.diagenode.com/files/products/kits/primer-indexes-for-tagmented-libraries_manual.pdf" target="_blank" title="Primer indexes for tagmented libraries - Manual">Primer indexes for tagmented libraries</a>.</p>',
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<p><small><strong>Figure 1. ChIPmentation sequencing profiles for Gata6</strong><br />Chromatin preparation and immunoprecipitation have been performed on hiPSCs (human induced Pluripotent Stem Cells) overexpressing Gata6 using the <a href="../../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq kit for TFs</a> (Cat. No. C01010055). Chromatin from 2,000,000 cells was used for the immunoprecipitation in combination with either anti-GATA6 antibody. The library preparation was performed with the <a href="../../p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a> (Cat. No. C01011030) and <a href="../../p/24-si-for-chipmentation">24 SI for ChIPmentation</a> (Cat. No. C01011031).</small></p>
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<p>The 8 SI for tagmented libraries includes 8 single primer indexes for multiplexing up to 8 samples. The primer indexes of the 8 SI Tagmented libraries are compatible with any tagmentation-based library preparation protocols, such as <a href="https://www.diagenode.com/en/categories/chromatin-ip-chipmentation">ChIPmentation</a>, <a href="https://www.diagenode.com/en/categories/atac-seq">ATAC-seq</a> or <a href="https://www.diagenode.com/en/categories/cutandtag">CUT&Tag</a> technologies.</p>
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<ul class="accordion" data-accordion="" style="margin-left: 0;">
<li class="accordion-navigation"><a style="background-color: white;" href="#workflow"> </a></li>
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<p><strong>Figure 1. Sequencing profiles of µChIPmentation libraries </strong><br />Chromatin preparation and immunoprecipitation have been performed on 10.000 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 SI for Tagmented libraries (Cat. No. C010111032) using K562 cells. The Diagenode antibodies targeting H3K4me3 (Cat. No. C15410003), H3K27ac (Cat. No. C15410196), H3K27me3 (Cat. No. C15410195) and H3K9me3 (Cat. No. C15410193) have been used.</p>
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<p><small><strong>Figure 1. ChIPmentation sequencing profiles for Gata6</strong><br />Chromatin preparation and immunoprecipitation have been performed on hiPSCs (human induced Pluripotent Stem Cells) overexpressing Gata6 using the <a href="../../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq kit for TFs</a> (Cat. No. C01010055). Chromatin from 2,000,000 cells was used for the immunoprecipitation in combination with either anti-GATA6 antibody. The library preparation was performed with the <a href="../../p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a> (Cat. No. C01011030) and <a href="../../p/24-si-for-chipmentation">24 SI for ChIPmentation</a> (Cat. No. C01011031).</small></p>
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<p>The <strong>24 SI for tagmented libraries</strong> includes 24 single primer indexes for multiplexing up to 24 samples. The primer indexes of the 24 SI Tagmented libraries are compatible with any <strong>tagmentation-based library preparation protocols</strong>, such as <a href="https://www.diagenode.com/en/categories/chromatin-ip-chipmentation">ChIPmentation</a>, <a href="https://www.diagenode.com/en/categories/atac-seq">ATAC-seq</a> or <a href="https://www.diagenode.com/en/categories/cutandtag">CUT&Tag</a> technologies.</p>
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<p><strong>Figure 1. Sequencing profiles of µChIPmentation libraries </strong><br />Chromatin preparation and immunoprecipitation have been performed on 10.000 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 SI for Tagmented libraries (Cat. No. C010111032) using K562 cells. The Diagenode antibodies targeting H3K4me3 (Cat. No. C15410003), H3K27ac (Cat. No. C15410196), H3K27me3 (Cat. No. C15410195) and H3K9me3 (Cat. No. C15410193) have been used.</p>
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<div class="small-12 medium-8 large-8 columns">
<div id="video"></div>
<h2>ChIPmentation for high-quality and fast <br />ChIP-seq library preparation</h2>
<p>Diagenode’s ChIPmentation technology, based on tagmentation, enables the integration of library preparation during ChIP itself using transposase and sequencing-compatible adaptors. Unlike standard library preparation techniques that require multi-step ligation, ChIPmentation incorporates an easier and shorter protocol.</p>
<p>Different protocols have been optimized bringing the solution for different needs:</p>
<ul class="no-bullet">
<li>✓ <strong>Low cell number</strong> – The kit <a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">µChIPmentation for Histones</a> enables to use as little as 10.000 cells per reaction New!</li>
<li>✓ <strong>Standard cell number</strong> – <a href="https://www.diagenode.com/en/p/chipmentation-kit-for-histones">ChIPmentation kit for Histones</a> - two complete protocols have been optimized: a manual procedure and an automated protocol on the IP-Star Compact Automated System.</li>
<li>✓<strong> Module for library prep using</strong> <strong>tagmentation</strong> – <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">Tag Kit for ChIPmentation</a> can be used with any ChIP protocol</li>
</ul>
<div class="extra-spaced">
<div id="video" align="center"><video width="400" height="250" autoplay="autoplay" muted="" loop="loop" controls="controls" src="https://www.diagenode.com/videos/chipmentation-dgo.mp4" frameborder="0" allowfullscreen="allowfullscreen"></video></div>
<div align="center"><a href="https://www.diagenode.com/pages/form-chipmentation" class="center alert radius button"><i class="fa fa-info"></i> Contact us</a></div>
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<div class="small-12 medium-4 large-4 columns"><center><a href="https://www.citeab.com/awards/2018/innovative-product-of-the-year" target="_blank"><img src="https://www.diagenode.com/emailing/images/citeab.png" width="224" height="200" /></a><br /><br />
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<div class="extra-spaced">
<h2>Benefits of the ChIPmentation system for optimal ChIP-seq</h2>
<ul class="nobullet">
<li><i class="fa fa-arrow-circle-right"></i> Ensure high quality sequencing data</li>
<li><i class="fa fa-arrow-circle-right"></i> Perform ChIP-seq on low input samples</li>
<li><i class="fa fa-arrow-circle-right"></i> Enjoy an easy and fast protocol</li>
<li><i class="fa fa-arrow-circle-right"></i> Benefit from the elimination of sequencing adaptor dimers</li>
<li><i class="fa fa-arrow-circle-right"></i> Automate on the IP-Star® to support standardization and save time</li>
</ul>
</div>
<div class="extra-spaced">
<ul class="accordion" data-accordion="" style="margin-left: 0; padding-left: 0;">
<li class="accordion-navigation"><a href="#more" style="color: #13b29c; background-color: transparent; display: inline; padding: 0; margin-left: 0;">ChIPmentation workflow - Read more</a>
<div id="more" class="content"><center><img src="https://www.diagenode.com/img/product/kits/chipmentation-for-histones-workflow.png" alt="Diagenode-ChIPmentation" caption="false" width="728" height="1024" /></center>
<div class="spaced">In traditional ChIP-seq library preparation, the ligation of the adaptors is performed after chromatin immunoprecipitation, in three steps. In the Diagenode ChIPmentation workflow, not only are the adaptors directly incorporated during ChIP, but also the main steps are automated on the Diagenode IP-Star®. The combination of direct adaptor incorporation and automation allows for higher sensitivity from low cell numbers and reproducibility of results.</div>
</div>
</li>
</ul>
</div>
<div class="extra-spaced">
<h2>Complete kits:</h2>
<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#v1" style="color: #13b29c;"><i class="fa fa-caret-right"></i> µChIPmentation for Histones</a>
<div id="v1" class="content">The Diagenode µChIPmentation Kit for Histones is optimized to perform ChIP-seq on as little as 10.000 cells from cell fixation to purified libraries.<br /><a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v2" style="color: #13b29c;"><i class="fa fa-caret-right"></i> ChIPmentation Kit for Histones </a>
<div id="v2" class="content">ChIPmentation is a ligation-free, tagmentation-based protocol that incorporates chromatin immunoprecipitation with NGS library preparation.<br /><a href="https://www.diagenode.com/en/p/chipmentation-kit-for-histones">Read more</a></div>
</li>
</ul>
<h2>Modules:</h2>
<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#v3" style="color: #13b29c;"><i class="fa fa-caret-right"></i> TAG Kit for ChIPmentation</a>
<div id="v3" class="content">The TAG Kit for ChIPmentation has been developed for researchers willing to perform ChIPmentation using their own validated ChIP protocol.<br /><a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">Read more</a></div>
</li>
</ul>
<h2>Primer indexes for tagmented libraries:</h2>
<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#v4" style="color: #13b29c;"><i class="fa fa-caret-right"></i> 24 SI for ChIPmentation</a>
<div id="v4" class="content">The 24 SI for ChIPmentation includes 24 single indexes compliting the TAG Kit for ChIPmentation.<br /><a href="https://www.diagenode.com/en/p/24-si-for-chipmentation">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v5" style="color: #13b29c;"><i class="fa fa-caret-right"></i> 24 SI for Tagmented libraries</a>
<div id="v5" class="content">The 24 SI for tagmented libraries includes 24 single primer indexes for multiplexing up to 24 samples. This set of indexes is compatible with any tagmentation-based library preparation protocols, such as ChIPmentation, ATAC-seq or CUT&Tag technologies.<br /><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v6" style="color: #13b29c;"><i class="fa fa-caret-right"></i> 24 UDI for Tagmented libraries - Set I</a>
<div id="v6" class="content">The 24 UDI (Unique dual indexes) for tagmented libraries – Set I provides combinations of barcodes where each barcode is uniquely attributed to one sample.<br /><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v7" style="color: #13b29c;"><i class="fa fa-caret-right"></i> 24 UDI for Tagmented libraries - Set II</a>
<div id="v7" class="content">The 24 UDI (Unique dual indexes) for tagmented libraries – Set II includes 24 primer pairs for unique dual-indexing and is designed to be combined with the 24 UDI for tagmented libraries – Set I, allowing multiplexing of up to 48 samples for sequencing on Illumina platforms.<br /><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v8" style="color: #13b29c;"><i class="fa fa-caret-right"></i> 24 UDI for Tagmented libraries - Set III</a>
<div id="v8" class="content">The 24 UDI (Unique dual indexes) for tagmented libraries – Set III includes 24 primer pairs for unique dual-indexing. It is designed to be used in combination with other sets of UDI (Set I, II and IV), allowing multiplexing of up to 96 samples for sequencing on Illumina platforms. <br /><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3">Read more</a></div>
</li>
<li class="accordion-navigation"><br />
<div id="v9" class="content">The 24 UDI (Unique dual indexes) for tagmented libraries – Set IV includes 24 primer pairs for unique dual-indexing. It is designed to be used in combination with other sets of UDI (Set I, II and III), allowing multiplexing of up to 96 samples for sequencing on Illumina platforms. <br /><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set4">Read more</a></div>
</li>
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<h2>Separately available products:</h2>
<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#v10" style="color: #13b29c;"><i class="fa fa-caret-right"></i> Tagmentase (Tn5 transposase) - loaded</a>
<div id="v10" class="content">Diagenode Tagmentase – loaded is a hyperactive Tn5 transposase preloaded with Illumina-compatible sequencing adapters.<br /><a href="https://www.diagenode.com/en/p/tagmentase-loaded-30">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v11" style="color: #13b29c;"><i class="fa fa-caret-right"></i> Tagmentase (Tn5 transposase) - unloaded</a>
<div id="v11" class="content">Diagenode Tagmentase is a hyperactive Tn5 transposase with the ability to cut DNA and insert sequences of interest into any target DNA in one step. This enzyme is not loaded with DNA oligos.<br /><a href="https://www.diagenode.com/en/p/tagmentase">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v12" style="color: #13b29c;"><i class="fa fa-caret-right"></i> Tagmentase Dilution Buffer</a>
<div id="v12" class="content">Diagenode Tagmentase Dilution Buffer is recommended for dilution of Tagmentase (Cat. No. C01070010) before or after transposome assembly.<br /><a href="https://www.diagenode.com/en/p/tagmentase-dilution-buffer">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v13" style="color: #13b29c;"><i class="fa fa-caret-right"></i> Tagmentation Buffer (1x)</a>
<div id="v13" class="content">Diagenode Tagmentation Buffer (1x) is the recommended reagent to perform any tagmentation reactions.<br /><a href="https://www.diagenode.com/en/p/tagmentation-buffer-1x-1ml">Read more</a></div>
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<p>Get the manual of <a href="https://www.diagenode.com/files/products/kits/primer-indexes-for-tagmented-libraries_manual.pdf" target="_blank" title="Primer indexes for tagmented libraries - Manual">Primer indexes for tagmented libraries</a>.</p>',
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'description' => '<p>One of our issues was that we could obtain only a limited number of cells, which is not enough for canonical ChIP-seq protocols. To solve this issue, we used the Diagenode ChIPmentation solution composed of iDeal ChIP-seq Kit for Transcription Factor, TAG Kit for ChIPmentation, and 24 SI for ChIPmentation. We performed ChIPmentation with IP-Star automated system for GATA6 in 2 million GATA6-overxpressing iPS cells. The result showed clear signal/noise ratio and was highly reproducible. This solution also worked in vitro differentiated definitive endoderm cells (data not shown).</p>
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<p><small><strong>Figure 1. ChIPmentation sequencing profiles for Gata6</strong><br />Chromatin preparation and immunoprecipitation have been performed on hiPSCs (human induced Pluripotent Stem Cells) overexpressing Gata6 using the <a href="../../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq kit for TFs</a> (Cat. No. C01010055). Chromatin from 2,000,000 cells was used for the immunoprecipitation in combination with either anti-GATA6 antibody. The library preparation was performed with the <a href="../../p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a> (Cat. No. C01011030) and <a href="../../p/24-si-for-chipmentation">24 SI for ChIPmentation</a> (Cat. No. C01011031).</small></p>
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<p>The 8 SI for tagmented libraries includes 8 single primer indexes for multiplexing up to 8 samples. The primer indexes of the 8 SI Tagmented libraries are compatible with any tagmentation-based library preparation protocols, such as <a href="https://www.diagenode.com/en/categories/chromatin-ip-chipmentation">ChIPmentation</a>, <a href="https://www.diagenode.com/en/categories/atac-seq">ATAC-seq</a> or <a href="https://www.diagenode.com/en/categories/cutandtag">CUT&Tag</a> technologies.</p>
<p>Additionally, the Unique Dual indexes for tagmented libraries are available. <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">Read more</a></p>',
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<ul class="accordion" data-accordion="" style="margin-left: 0;">
<li class="accordion-navigation"><a style="background-color: white;" href="#workflow"> </a></li>
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<p><strong>Figure 1. Sequencing profiles of µChIPmentation libraries </strong><br />Chromatin preparation and immunoprecipitation have been performed on 10.000 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 SI for Tagmented libraries (Cat. No. C010111032) using K562 cells. The Diagenode antibodies targeting H3K4me3 (Cat. No. C15410003), H3K27ac (Cat. No. C15410196), H3K27me3 (Cat. No. C15410195) and H3K9me3 (Cat. No. C15410193) have been used.</p>
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<div class="extra-spaced"></div>
<div class="row">
<div class="small-12 columns">
<p><small><strong>Region 1</strong></small></p>
<center>
<p><img src="../../img/product/kits/chipmentation-gata6-region1.png" /></p>
</center></div>
<div class="small-12 columns">
<p><small><strong>Region 2</strong></small></p>
<center><img src="../../img/product/kits/chipmentation-gata6-region2.png" /></center></div>
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<div class="extra-spaced"></div>
<div class="row">
<div class="small-12 columns">
<p><small><strong>Figure 1. ChIPmentation sequencing profiles for Gata6</strong><br />Chromatin preparation and immunoprecipitation have been performed on hiPSCs (human induced Pluripotent Stem Cells) overexpressing Gata6 using the <a href="../../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq kit for TFs</a> (Cat. No. C01010055). Chromatin from 2,000,000 cells was used for the immunoprecipitation in combination with either anti-GATA6 antibody. The library preparation was performed with the <a href="../../p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a> (Cat. No. C01011030) and <a href="../../p/24-si-for-chipmentation">24 SI for ChIPmentation</a> (Cat. No. C01011031).</small></p>
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</div><cite>Takahiro Suzuki, Ph.D., Senior Research Scientist, Cellular Function Conversion Technology Team, RIKEN Center for Integrative Medical Sciences, Yokohama, Japan</cite></blockquote>
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<p><small><strong>Figure 1. ChIPmentation sequencing profiles for Gata6</strong><br />Chromatin preparation and immunoprecipitation have been performed on hiPSCs (human induced Pluripotent Stem Cells) overexpressing Gata6 using the <a href="../../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq kit for TFs</a> (Cat. No. C01010055). Chromatin from 2,000,000 cells was used for the immunoprecipitation in combination with either anti-GATA6 antibody. The library preparation was performed with the <a href="../../p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a> (Cat. No. C01011030) and <a href="../../p/24-si-for-chipmentation">24 SI for ChIPmentation</a> (Cat. No. C01011031).</small></p>
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<p><strong>Figure 1. Sequencing profiles of µChIPmentation libraries </strong><br />Chromatin preparation and immunoprecipitation have been performed on 10.000 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 SI for Tagmented libraries (Cat. No. C010111032) using K562 cells. The Diagenode antibodies targeting H3K4me3 (Cat. No. C15410003), H3K27ac (Cat. No. C15410196), H3K27me3 (Cat. No. C15410195) and H3K9me3 (Cat. No. C15410193) have been used.</p>
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<p>The <strong>24 SI for tagmented libraries</strong> includes 24 single primer indexes for multiplexing up to 24 samples. The primer indexes of the 24 SI Tagmented libraries are compatible with any <strong>tagmentation-based library preparation protocols</strong>, such as <a href="https://www.diagenode.com/en/categories/chromatin-ip-chipmentation">ChIPmentation</a>, <a href="https://www.diagenode.com/en/categories/atac-seq">ATAC-seq</a> or <a href="https://www.diagenode.com/en/categories/cutandtag">CUT&Tag</a> technologies.</p>
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<p><strong>Figure 1. Sequencing profiles of µChIPmentation libraries </strong><br />Chromatin preparation and immunoprecipitation have been performed on 10.000 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 SI for Tagmented libraries (Cat. No. C010111032) using K562 cells. The Diagenode antibodies targeting H3K4me3 (Cat. No. C15410003), H3K27ac (Cat. No. C15410196), H3K27me3 (Cat. No. C15410195) and H3K9me3 (Cat. No. C15410193) have been used.</p>
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<div id="video"></div>
<h2>ChIPmentation for high-quality and fast <br />ChIP-seq library preparation</h2>
<p>Diagenode’s ChIPmentation technology, based on tagmentation, enables the integration of library preparation during ChIP itself using transposase and sequencing-compatible adaptors. Unlike standard library preparation techniques that require multi-step ligation, ChIPmentation incorporates an easier and shorter protocol.</p>
<p>Different protocols have been optimized bringing the solution for different needs:</p>
<ul class="no-bullet">
<li>✓ <strong>Low cell number</strong> – The kit <a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">µChIPmentation for Histones</a> enables to use as little as 10.000 cells per reaction New!</li>
<li>✓ <strong>Standard cell number</strong> – <a href="https://www.diagenode.com/en/p/chipmentation-kit-for-histones">ChIPmentation kit for Histones</a> - two complete protocols have been optimized: a manual procedure and an automated protocol on the IP-Star Compact Automated System.</li>
<li>✓<strong> Module for library prep using</strong> <strong>tagmentation</strong> – <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">Tag Kit for ChIPmentation</a> can be used with any ChIP protocol</li>
</ul>
<div class="extra-spaced">
<div id="video" align="center"><video width="400" height="250" autoplay="autoplay" muted="" loop="loop" controls="controls" src="https://www.diagenode.com/videos/chipmentation-dgo.mp4" frameborder="0" allowfullscreen="allowfullscreen"></video></div>
<div align="center"><a href="https://www.diagenode.com/pages/form-chipmentation" class="center alert radius button"><i class="fa fa-info"></i> Contact us</a></div>
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<div class="small-12 medium-4 large-4 columns"><center><a href="https://www.citeab.com/awards/2018/innovative-product-of-the-year" target="_blank"><img src="https://www.diagenode.com/emailing/images/citeab.png" width="224" height="200" /></a><br /><br />
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<div class="extra-spaced">
<h2>Benefits of the ChIPmentation system for optimal ChIP-seq</h2>
<ul class="nobullet">
<li><i class="fa fa-arrow-circle-right"></i> Ensure high quality sequencing data</li>
<li><i class="fa fa-arrow-circle-right"></i> Perform ChIP-seq on low input samples</li>
<li><i class="fa fa-arrow-circle-right"></i> Enjoy an easy and fast protocol</li>
<li><i class="fa fa-arrow-circle-right"></i> Benefit from the elimination of sequencing adaptor dimers</li>
<li><i class="fa fa-arrow-circle-right"></i> Automate on the IP-Star® to support standardization and save time</li>
</ul>
</div>
<div class="extra-spaced">
<ul class="accordion" data-accordion="" style="margin-left: 0; padding-left: 0;">
<li class="accordion-navigation"><a href="#more" style="color: #13b29c; background-color: transparent; display: inline; padding: 0; margin-left: 0;">ChIPmentation workflow - Read more</a>
<div id="more" class="content"><center><img src="https://www.diagenode.com/img/product/kits/chipmentation-for-histones-workflow.png" alt="Diagenode-ChIPmentation" caption="false" width="728" height="1024" /></center>
<div class="spaced">In traditional ChIP-seq library preparation, the ligation of the adaptors is performed after chromatin immunoprecipitation, in three steps. In the Diagenode ChIPmentation workflow, not only are the adaptors directly incorporated during ChIP, but also the main steps are automated on the Diagenode IP-Star®. The combination of direct adaptor incorporation and automation allows for higher sensitivity from low cell numbers and reproducibility of results.</div>
</div>
</li>
</ul>
</div>
<div class="extra-spaced">
<h2>Complete kits:</h2>
<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#v1" style="color: #13b29c;"><i class="fa fa-caret-right"></i> µChIPmentation for Histones</a>
<div id="v1" class="content">The Diagenode µChIPmentation Kit for Histones is optimized to perform ChIP-seq on as little as 10.000 cells from cell fixation to purified libraries.<br /><a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v2" style="color: #13b29c;"><i class="fa fa-caret-right"></i> ChIPmentation Kit for Histones </a>
<div id="v2" class="content">ChIPmentation is a ligation-free, tagmentation-based protocol that incorporates chromatin immunoprecipitation with NGS library preparation.<br /><a href="https://www.diagenode.com/en/p/chipmentation-kit-for-histones">Read more</a></div>
</li>
</ul>
<h2>Modules:</h2>
<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#v3" style="color: #13b29c;"><i class="fa fa-caret-right"></i> TAG Kit for ChIPmentation</a>
<div id="v3" class="content">The TAG Kit for ChIPmentation has been developed for researchers willing to perform ChIPmentation using their own validated ChIP protocol.<br /><a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">Read more</a></div>
</li>
</ul>
<h2>Primer indexes for tagmented libraries:</h2>
<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#v4" style="color: #13b29c;"><i class="fa fa-caret-right"></i> 24 SI for ChIPmentation</a>
<div id="v4" class="content">The 24 SI for ChIPmentation includes 24 single indexes compliting the TAG Kit for ChIPmentation.<br /><a href="https://www.diagenode.com/en/p/24-si-for-chipmentation">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v5" style="color: #13b29c;"><i class="fa fa-caret-right"></i> 24 SI for Tagmented libraries</a>
<div id="v5" class="content">The 24 SI for tagmented libraries includes 24 single primer indexes for multiplexing up to 24 samples. This set of indexes is compatible with any tagmentation-based library preparation protocols, such as ChIPmentation, ATAC-seq or CUT&Tag technologies.<br /><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v6" style="color: #13b29c;"><i class="fa fa-caret-right"></i> 24 UDI for Tagmented libraries - Set I</a>
<div id="v6" class="content">The 24 UDI (Unique dual indexes) for tagmented libraries – Set I provides combinations of barcodes where each barcode is uniquely attributed to one sample.<br /><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v7" style="color: #13b29c;"><i class="fa fa-caret-right"></i> 24 UDI for Tagmented libraries - Set II</a>
<div id="v7" class="content">The 24 UDI (Unique dual indexes) for tagmented libraries – Set II includes 24 primer pairs for unique dual-indexing and is designed to be combined with the 24 UDI for tagmented libraries – Set I, allowing multiplexing of up to 48 samples for sequencing on Illumina platforms.<br /><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v8" style="color: #13b29c;"><i class="fa fa-caret-right"></i> 24 UDI for Tagmented libraries - Set III</a>
<div id="v8" class="content">The 24 UDI (Unique dual indexes) for tagmented libraries – Set III includes 24 primer pairs for unique dual-indexing. It is designed to be used in combination with other sets of UDI (Set I, II and IV), allowing multiplexing of up to 96 samples for sequencing on Illumina platforms. <br /><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3">Read more</a></div>
</li>
<li class="accordion-navigation"><br />
<div id="v9" class="content">The 24 UDI (Unique dual indexes) for tagmented libraries – Set IV includes 24 primer pairs for unique dual-indexing. It is designed to be used in combination with other sets of UDI (Set I, II and III), allowing multiplexing of up to 96 samples for sequencing on Illumina platforms. <br /><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set4">Read more</a></div>
</li>
</ul>
<h2>Separately available products:</h2>
<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#v10" style="color: #13b29c;"><i class="fa fa-caret-right"></i> Tagmentase (Tn5 transposase) - loaded</a>
<div id="v10" class="content">Diagenode Tagmentase – loaded is a hyperactive Tn5 transposase preloaded with Illumina-compatible sequencing adapters.<br /><a href="https://www.diagenode.com/en/p/tagmentase-loaded-30">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v11" style="color: #13b29c;"><i class="fa fa-caret-right"></i> Tagmentase (Tn5 transposase) - unloaded</a>
<div id="v11" class="content">Diagenode Tagmentase is a hyperactive Tn5 transposase with the ability to cut DNA and insert sequences of interest into any target DNA in one step. This enzyme is not loaded with DNA oligos.<br /><a href="https://www.diagenode.com/en/p/tagmentase">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v12" style="color: #13b29c;"><i class="fa fa-caret-right"></i> Tagmentase Dilution Buffer</a>
<div id="v12" class="content">Diagenode Tagmentase Dilution Buffer is recommended for dilution of Tagmentase (Cat. No. C01070010) before or after transposome assembly.<br /><a href="https://www.diagenode.com/en/p/tagmentase-dilution-buffer">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v13" style="color: #13b29c;"><i class="fa fa-caret-right"></i> Tagmentation Buffer (1x)</a>
<div id="v13" class="content">Diagenode Tagmentation Buffer (1x) is the recommended reagent to perform any tagmentation reactions.<br /><a href="https://www.diagenode.com/en/p/tagmentation-buffer-1x-1ml">Read more</a></div>
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<p>Get the manual of <a href="https://www.diagenode.com/files/products/kits/primer-indexes-for-tagmented-libraries_manual.pdf" target="_blank" title="Primer indexes for tagmented libraries - Manual">Primer indexes for tagmented libraries</a>.</p>',
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<p><small><strong>Figure 1. ChIPmentation sequencing profiles for Gata6</strong><br />Chromatin preparation and immunoprecipitation have been performed on hiPSCs (human induced Pluripotent Stem Cells) overexpressing Gata6 using the <a href="../../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq kit for TFs</a> (Cat. No. C01010055). Chromatin from 2,000,000 cells was used for the immunoprecipitation in combination with either anti-GATA6 antibody. The library preparation was performed with the <a href="../../p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a> (Cat. No. C01011030) and <a href="../../p/24-si-for-chipmentation">24 SI for ChIPmentation</a> (Cat. No. C01011031).</small></p>
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<p>The 8 SI for tagmented libraries includes 8 single primer indexes for multiplexing up to 8 samples. The primer indexes of the 8 SI Tagmented libraries are compatible with any tagmentation-based library preparation protocols, such as <a href="https://www.diagenode.com/en/categories/chromatin-ip-chipmentation">ChIPmentation</a>, <a href="https://www.diagenode.com/en/categories/atac-seq">ATAC-seq</a> or <a href="https://www.diagenode.com/en/categories/cutandtag">CUT&Tag</a> technologies.</p>
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<ul class="accordion" data-accordion="" style="margin-left: 0;">
<li class="accordion-navigation"><a style="background-color: white;" href="#workflow"> </a></li>
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<p><strong>Figure 1. Sequencing profiles of µChIPmentation libraries </strong><br />Chromatin preparation and immunoprecipitation have been performed on 10.000 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 SI for Tagmented libraries (Cat. No. C010111032) using K562 cells. The Diagenode antibodies targeting H3K4me3 (Cat. No. C15410003), H3K27ac (Cat. No. C15410196), H3K27me3 (Cat. No. C15410195) and H3K9me3 (Cat. No. C15410193) have been used.</p>
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<p><small><strong>Figure 1. ChIPmentation sequencing profiles for Gata6</strong><br />Chromatin preparation and immunoprecipitation have been performed on hiPSCs (human induced Pluripotent Stem Cells) overexpressing Gata6 using the <a href="../../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq kit for TFs</a> (Cat. No. C01010055). Chromatin from 2,000,000 cells was used for the immunoprecipitation in combination with either anti-GATA6 antibody. The library preparation was performed with the <a href="../../p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a> (Cat. No. C01011030) and <a href="../../p/24-si-for-chipmentation">24 SI for ChIPmentation</a> (Cat. No. C01011031).</small></p>
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<p>The <strong>24 SI for tagmented libraries</strong> includes 24 single primer indexes for multiplexing up to 24 samples. The primer indexes of the 24 SI Tagmented libraries are compatible with any <strong>tagmentation-based library preparation protocols</strong>, such as <a href="https://www.diagenode.com/en/categories/chromatin-ip-chipmentation">ChIPmentation</a>, <a href="https://www.diagenode.com/en/categories/atac-seq">ATAC-seq</a> or <a href="https://www.diagenode.com/en/categories/cutandtag">CUT&Tag</a> technologies.</p>
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<h2>ChIPmentation for high-quality and fast <br />ChIP-seq library preparation</h2>
<p>Diagenode’s ChIPmentation technology, based on tagmentation, enables the integration of library preparation during ChIP itself using transposase and sequencing-compatible adaptors. Unlike standard library preparation techniques that require multi-step ligation, ChIPmentation incorporates an easier and shorter protocol.</p>
<p>Different protocols have been optimized bringing the solution for different needs:</p>
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<li>✓ <strong>Low cell number</strong> – The kit <a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">µChIPmentation for Histones</a> enables to use as little as 10.000 cells per reaction New!</li>
<li>✓ <strong>Standard cell number</strong> – <a href="https://www.diagenode.com/en/p/chipmentation-kit-for-histones">ChIPmentation kit for Histones</a> - two complete protocols have been optimized: a manual procedure and an automated protocol on the IP-Star Compact Automated System.</li>
<li>✓<strong> Module for library prep using</strong> <strong>tagmentation</strong> – <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">Tag Kit for ChIPmentation</a> can be used with any ChIP protocol</li>
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<div class="extra-spaced">
<h2>Benefits of the ChIPmentation system for optimal ChIP-seq</h2>
<ul class="nobullet">
<li><i class="fa fa-arrow-circle-right"></i> Ensure high quality sequencing data</li>
<li><i class="fa fa-arrow-circle-right"></i> Perform ChIP-seq on low input samples</li>
<li><i class="fa fa-arrow-circle-right"></i> Enjoy an easy and fast protocol</li>
<li><i class="fa fa-arrow-circle-right"></i> Benefit from the elimination of sequencing adaptor dimers</li>
<li><i class="fa fa-arrow-circle-right"></i> Automate on the IP-Star® to support standardization and save time</li>
</ul>
</div>
<div class="extra-spaced">
<ul class="accordion" data-accordion="" style="margin-left: 0; padding-left: 0;">
<li class="accordion-navigation"><a href="#more" style="color: #13b29c; background-color: transparent; display: inline; padding: 0; margin-left: 0;">ChIPmentation workflow - Read more</a>
<div id="more" class="content"><center><img src="https://www.diagenode.com/img/product/kits/chipmentation-for-histones-workflow.png" alt="Diagenode-ChIPmentation" caption="false" width="728" height="1024" /></center>
<div class="spaced">In traditional ChIP-seq library preparation, the ligation of the adaptors is performed after chromatin immunoprecipitation, in three steps. In the Diagenode ChIPmentation workflow, not only are the adaptors directly incorporated during ChIP, but also the main steps are automated on the Diagenode IP-Star®. The combination of direct adaptor incorporation and automation allows for higher sensitivity from low cell numbers and reproducibility of results.</div>
</div>
</li>
</ul>
</div>
<div class="extra-spaced">
<h2>Complete kits:</h2>
<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#v1" style="color: #13b29c;"><i class="fa fa-caret-right"></i> µChIPmentation for Histones</a>
<div id="v1" class="content">The Diagenode µChIPmentation Kit for Histones is optimized to perform ChIP-seq on as little as 10.000 cells from cell fixation to purified libraries.<br /><a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v2" style="color: #13b29c;"><i class="fa fa-caret-right"></i> ChIPmentation Kit for Histones </a>
<div id="v2" class="content">ChIPmentation is a ligation-free, tagmentation-based protocol that incorporates chromatin immunoprecipitation with NGS library preparation.<br /><a href="https://www.diagenode.com/en/p/chipmentation-kit-for-histones">Read more</a></div>
</li>
</ul>
<h2>Modules:</h2>
<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#v3" style="color: #13b29c;"><i class="fa fa-caret-right"></i> TAG Kit for ChIPmentation</a>
<div id="v3" class="content">The TAG Kit for ChIPmentation has been developed for researchers willing to perform ChIPmentation using their own validated ChIP protocol.<br /><a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">Read more</a></div>
</li>
</ul>
<h2>Primer indexes for tagmented libraries:</h2>
<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#v4" style="color: #13b29c;"><i class="fa fa-caret-right"></i> 24 SI for ChIPmentation</a>
<div id="v4" class="content">The 24 SI for ChIPmentation includes 24 single indexes compliting the TAG Kit for ChIPmentation.<br /><a href="https://www.diagenode.com/en/p/24-si-for-chipmentation">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v5" style="color: #13b29c;"><i class="fa fa-caret-right"></i> 24 SI for Tagmented libraries</a>
<div id="v5" class="content">The 24 SI for tagmented libraries includes 24 single primer indexes for multiplexing up to 24 samples. This set of indexes is compatible with any tagmentation-based library preparation protocols, such as ChIPmentation, ATAC-seq or CUT&Tag technologies.<br /><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v6" style="color: #13b29c;"><i class="fa fa-caret-right"></i> 24 UDI for Tagmented libraries - Set I</a>
<div id="v6" class="content">The 24 UDI (Unique dual indexes) for tagmented libraries – Set I provides combinations of barcodes where each barcode is uniquely attributed to one sample.<br /><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v7" style="color: #13b29c;"><i class="fa fa-caret-right"></i> 24 UDI for Tagmented libraries - Set II</a>
<div id="v7" class="content">The 24 UDI (Unique dual indexes) for tagmented libraries – Set II includes 24 primer pairs for unique dual-indexing and is designed to be combined with the 24 UDI for tagmented libraries – Set I, allowing multiplexing of up to 48 samples for sequencing on Illumina platforms.<br /><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v8" style="color: #13b29c;"><i class="fa fa-caret-right"></i> 24 UDI for Tagmented libraries - Set III</a>
<div id="v8" class="content">The 24 UDI (Unique dual indexes) for tagmented libraries – Set III includes 24 primer pairs for unique dual-indexing. It is designed to be used in combination with other sets of UDI (Set I, II and IV), allowing multiplexing of up to 96 samples for sequencing on Illumina platforms. <br /><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3">Read more</a></div>
</li>
<li class="accordion-navigation"><br />
<div id="v9" class="content">The 24 UDI (Unique dual indexes) for tagmented libraries – Set IV includes 24 primer pairs for unique dual-indexing. It is designed to be used in combination with other sets of UDI (Set I, II and III), allowing multiplexing of up to 96 samples for sequencing on Illumina platforms. <br /><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set4">Read more</a></div>
</li>
</ul>
<h2>Separately available products:</h2>
<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#v10" style="color: #13b29c;"><i class="fa fa-caret-right"></i> Tagmentase (Tn5 transposase) - loaded</a>
<div id="v10" class="content">Diagenode Tagmentase – loaded is a hyperactive Tn5 transposase preloaded with Illumina-compatible sequencing adapters.<br /><a href="https://www.diagenode.com/en/p/tagmentase-loaded-30">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v11" style="color: #13b29c;"><i class="fa fa-caret-right"></i> Tagmentase (Tn5 transposase) - unloaded</a>
<div id="v11" class="content">Diagenode Tagmentase is a hyperactive Tn5 transposase with the ability to cut DNA and insert sequences of interest into any target DNA in one step. This enzyme is not loaded with DNA oligos.<br /><a href="https://www.diagenode.com/en/p/tagmentase">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v12" style="color: #13b29c;"><i class="fa fa-caret-right"></i> Tagmentase Dilution Buffer</a>
<div id="v12" class="content">Diagenode Tagmentase Dilution Buffer is recommended for dilution of Tagmentase (Cat. No. C01070010) before or after transposome assembly.<br /><a href="https://www.diagenode.com/en/p/tagmentase-dilution-buffer">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v13" style="color: #13b29c;"><i class="fa fa-caret-right"></i> Tagmentation Buffer (1x)</a>
<div id="v13" class="content">Diagenode Tagmentation Buffer (1x) is the recommended reagent to perform any tagmentation reactions.<br /><a href="https://www.diagenode.com/en/p/tagmentation-buffer-1x-1ml">Read more</a></div>
</li>
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<h3>Features:</h3>
<ul>
<li>Compatibility: <strong>tagmentation-based library</strong> preparation protocols</li>
<li>Flexibility: <strong>Single</strong> and <strong>unique dual indexing</strong> available</li>
<li>Multiplexing capacity: up to <strong>72 samples</strong> (with UDI)</li>
<li>Identification and <strong>filtering of index hopping</strong> – using the UDI</li>
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<p>Get the manual of <a href="https://www.diagenode.com/files/products/kits/primer-indexes-for-tagmented-libraries_manual.pdf" target="_blank" title="Primer indexes for tagmented libraries - Manual">Primer indexes for tagmented libraries</a>.</p>',
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<div class="extra-spaced"></div>
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<p><small><strong>Region 1</strong></small></p>
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<p><small><strong>Region 2</strong></small></p>
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<p><small><strong>Figure 1. ChIPmentation sequencing profiles for Gata6</strong><br />Chromatin preparation and immunoprecipitation have been performed on hiPSCs (human induced Pluripotent Stem Cells) overexpressing Gata6 using the <a href="../../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq kit for TFs</a> (Cat. No. C01010055). Chromatin from 2,000,000 cells was used for the immunoprecipitation in combination with either anti-GATA6 antibody. The library preparation was performed with the <a href="../../p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a> (Cat. No. C01011030) and <a href="../../p/24-si-for-chipmentation">24 SI for ChIPmentation</a> (Cat. No. C01011031).</small></p>
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<p>The 8 SI for tagmented libraries includes 8 single primer indexes for multiplexing up to 8 samples. The primer indexes of the 8 SI Tagmented libraries are compatible with any tagmentation-based library preparation protocols, such as <a href="https://www.diagenode.com/en/categories/chromatin-ip-chipmentation">ChIPmentation</a>, <a href="https://www.diagenode.com/en/categories/atac-seq">ATAC-seq</a> or <a href="https://www.diagenode.com/en/categories/cutandtag">CUT&Tag</a> technologies.</p>
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<p><strong>Figure 1. Sequencing profiles of µChIPmentation libraries </strong><br />Chromatin preparation and immunoprecipitation have been performed on 10.000 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 SI for Tagmented libraries (Cat. No. C010111032) using K562 cells. The Diagenode antibodies targeting H3K4me3 (Cat. No. C15410003), H3K27ac (Cat. No. C15410196), H3K27me3 (Cat. No. C15410195) and H3K9me3 (Cat. No. C15410193) have been used.</p>
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$featured_testimonials = '<blockquote><span class="label-green" style="margin-bottom:16px;margin-left:-22px">TESTIMONIAL</span><p>One of our issues was that we could obtain only a limited number of cells, which is not enough for canonical ChIP-seq protocols. To solve this issue, we used the Diagenode ChIPmentation solution composed of iDeal ChIP-seq Kit for Transcription Factor, TAG Kit for ChIPmentation, and 24 SI for ChIPmentation. We performed ChIPmentation with IP-Star automated system for GATA6 in 2 million GATA6-overxpressing iPS cells. The result showed clear signal/noise ratio and was highly reproducible. This solution also worked in vitro differentiated definitive endoderm cells (data not shown).</p>
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<p><small><strong>Figure 1. ChIPmentation sequencing profiles for Gata6</strong><br />Chromatin preparation and immunoprecipitation have been performed on hiPSCs (human induced Pluripotent Stem Cells) overexpressing Gata6 using the <a href="../../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq kit for TFs</a> (Cat. No. C01010055). Chromatin from 2,000,000 cells was used for the immunoprecipitation in combination with either anti-GATA6 antibody. The library preparation was performed with the <a href="../../p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a> (Cat. No. C01011030) and <a href="../../p/24-si-for-chipmentation">24 SI for ChIPmentation</a> (Cat. No. C01011031).</small></p>
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</div><cite>Takahiro Suzuki, Ph.D., Senior Research Scientist, Cellular Function Conversion Technology Team, RIKEN Center for Integrative Medical Sciences, Yokohama, Japan</cite></blockquote>
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'description' => '<p>One of our issues was that we could obtain only a limited number of cells, which is not enough for canonical ChIP-seq protocols. To solve this issue, we used the Diagenode ChIPmentation solution composed of iDeal ChIP-seq Kit for Transcription Factor, TAG Kit for ChIPmentation, and 24 SI for ChIPmentation. We performed ChIPmentation with IP-Star automated system for GATA6 in 2 million GATA6-overxpressing iPS cells. The result showed clear signal/noise ratio and was highly reproducible. This solution also worked in vitro differentiated definitive endoderm cells (data not shown).</p>
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<p><small><strong>Figure 1. ChIPmentation sequencing profiles for Gata6</strong><br />Chromatin preparation and immunoprecipitation have been performed on hiPSCs (human induced Pluripotent Stem Cells) overexpressing Gata6 using the <a href="../../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq kit for TFs</a> (Cat. No. C01010055). Chromatin from 2,000,000 cells was used for the immunoprecipitation in combination with either anti-GATA6 antibody. The library preparation was performed with the <a href="../../p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a> (Cat. No. C01011030) and <a href="../../p/24-si-for-chipmentation">24 SI for ChIPmentation</a> (Cat. No. C01011031).</small></p>
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'description' => '<p><span>How cell-extrinsic niche-related and cell-intrinsic cues drive lineage specification of hematopoietic multipotent progenitors (MPPs) in the bone marrow (BM) is partly understood. We show that CXCR4 signaling strength regulates localization and fate of MPPs. In mice phenocopying the BM myeloid skewing of patients with WHIM Syndrome (WS), a rare immunodeficiency caused by gain-of-function </span><em>CXCR4</em><span><span> </span>mutations, enhanced mTOR signaling and overactive Oxphos metabolism were associated with myeloid rewiring of lymphoid-primed MPPs (or MPP4). Fate decision of MPP4 was also affected by molecular changes established at the MPP1 level. Mutant MPP4 displayed altered BM localization relative to peri-arteriolar structures, suggesting that extrinsic cues contribute to their myeloid skewing. Chronic treatment with CXCR4 antagonist AMD3100 or mTOR inhibitor Rapamycin rescued lymphoid capacities of mutant MPP4, demonstrating a pivotal role for the CXCR4-mTOR axis in regulating MPP4 fate. Our study thus provides mechanistic insights into how CXCR4 signaling regulates the lymphoid potential of MPPs.</span></p>',
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