RFXAP (UniProtKB/Swiss-Prot entry O00287) is part of the RFX complex that binds to the X-box of MHC II promoters. The RFX complex consists of at least 3 different subunits; RFXAP, RFX5 and RFX-B/RFXANK; with each subunit representing a separate complementation group. RFX forms cooperative DNA binding complexes with X2BP and CBF/NF-Y. RFX associates with CIITA to form an active transcriptional complex. Defects in RFXAP can cause bare lymphocyte syndrome type II (BLS II); also known as hereditary MHC class II deficiency or HLA class II-deficient combined immunodeficiency.
RFX-AP Antibody
Catalog Number
Format
Price
Polyclonal antibody raised in rabbit against RFXAP (Regulatory factor X-associated protein), using the recombinant protein.
| Lot | 001 |
|---|---|
| Concentration | 1.0 µg/µl |
| Species reactivity | Human |
| Type | Polyclonal |
| Purity | Affinity purified |
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ChIP * | 5-7 μg/IP | Fig 1 |
* Please note that of the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 μg per IP.
- Validation Data
Figure 1 ChIP results obtained with the Diagenode antibody directed against RFXAP
ChIP assays were performed using NALM cells (a cell line derived from human pre-B leukemia), the Diagenode antibody against RFXAP (cat. No. pAb-061-050) and optimized primer sets for PCR. Sheared chromatin from 2 million cells and respectively 5 and 7 μg of antibody were used per ChIP experiment. An anti-flag antibody (lane 2) was used as negative IP control. Figure 1 shows the result of the end-point PCR with primers for HLA_DRA, used as positive control (lower panel) and for HLA_DOB, used a negative PCR control (upper panel). - Publications
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