HDAC3 (UniProt/Swiss-Prot entry O15379) catalyses the deacetylation of lysine residues in the N-terminal part of the core histones (H2A, H2B, H3 and H4). Acetylation and deacetylation of these highly conserved lysine residues is important for the control of gene expression and HDAC activity is associated with gene repression. Histone deacetylation is established by the formation of large multiprotein complexes. HDAC3 may bind to the zinc-finger transcription factor YY1, thereby regulating transcription. It is also able to modulate cell growth and apoptosis through the interaction with p53 and is thought to be essential for the repression of the POU1F1 transcription factor.
HDAC3 Antibody
Catalog Number
Format
Price
Alternative names: HD3, RPD3, RPD3-2, SMAP45
Monoclonal antibody raised in mouse against human HDAC3 (Histone deacetylase 3), using a KLH-conjugated synthetic peptide containing a sequence from the C-terminal region of the protein.
| Lot | 001 |
|---|---|
| Concentration | 2.0 µg/µl |
| Species reactivity | Human |
| Type | Monoclonal |
| Purity | Protein A purified monoclonal antibody |
| Host | Mouse |
| Storage Conditions | Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS containing 0.05% azide and 0.05% ProClin 300. |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| Immunofluorescence | 1:500 | Fig 1 |
- Validation Data
Figure 1. Immunofluorescence using the Diagenode monoclonal antibody directed against HDAC3
HeLa cells were stained with the Diagenode antibody against HDAC3 (Cat. No. C15200145) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the HDAC3 antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right. - Publications
How to properly cite our product/service in your work
We strongly recommend using this: HDAC3 Antibody (Hologic Diagenode Cat# C15200145 Lot# 001). Click here to copy to clipboard.
Using our products or services in your publication? Let us know!