The di-methylated K18 on histone H3 is a seemingly transient post-translational modification. H3K18 is better known to be acetylated, and occasionally mono-methylated. Suv39h1, a well-studied histone methyltransferase seems to be responsible for the transition of acetylation and methylation at this H3 modification site. The di-methylated K18 on H3 seems to be associated with embryological development and possibly implantation.
H3K18me2 Antibody
Catalog Number
Format
Price
Polyclonal antibody raised in rabbit against Histone H3 (dimethyl Lys18), using a KLH-conjugated synthetic peptide.
| Lot | 001 |
|---|---|
| Concentration | 0.79 μg/μl |
| Species reactivity | Human, mouse, C. elegans, rat, chicken, Xenopus, Drosophila, plant |
| Type | Polyclonal |
| Purity | Affinity purified |
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution * | References |
|---|---|---|
| ChIP | 2-5 μg/million cells | Fig 1 |
| Immunofluorescence | 1:500 | Fig 2 |
| Western Blotting | 1:500 | Fig 4, 5 |
| Immunochemistry | 1:100 | |
| Dot Blotting | 1:1,000 | Fig 3 |
- Validation Data
Figure 1. ChIP
Chromatin Immunoprecipitation of H3K18me2 antibody. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2 μg of H3K18me2 antibody and 20 μl of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using real-time PCR and normalized to the input chromatin.
Figure 2. Immunofluorescence
Immunofluorescence of H3K18me2 antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody used at a 1:500 dilution for 1 h at RT. Secondary antibody: Dylight 488 secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H3K18me2 is nuclear and chromosomal. Staining: Histone H3K18me2 is expressed in green, nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight 594 (red).
Figure 3. Dot Blot
Dot Blot of H3K18me2 antibody. Lane 1: K18 unmodified. Lane 2: K18Me2. Lane 3: K18Me3. Lane 4: K18ac. Lane 5: K18Me1. Load: 0.1, 0.3, and 1 picomoles of peptide. Primary antibody used at a 1:1,000 dilution for 45 min at 4°C. Secondary antibody: DylightTM488 rabbit secondary antibody at 1:10,000 for 45 min at RT.
Figure 4. Western Blot
Western Blot of H3K18me2 antibody. 30 μg of C. elegans embryo lysate. Primary antibody used at a 1:500 dilution overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~15 kDa. Other band(s): None.
Figure 5. Western Blot
Western Blot of H3K18me2 antibody. 30 μg of NIH-3T3 histone extract. Primary antibody used at a 1:500 dilution overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~15 kDa. Other band(s): None. - Publications
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