The D-Plex small RNA-seq SI library construct bears the TruSeq (Illumina) Small RNA adapters. In case of a multiplexing scenario, it is therefore recommended to submit the D-Plex libraries as TruSeq small RNA libraries to your sequencing provider. Further details are provided in the D-Plex Single Indexes manual.
D-Plex Single Indexes for Illumina - Set A
Catalog Number
Format
Price
C05030010
24 rxns
$420.00
D-Plex Single Indexes Module - Set A includes PCR primers with 24 barcodes (i7 index) for library multiplexing with the D-Plex Small RNA-seq Kit.
These single indexes (SI) were designed and validated to fit the D-Plex technology for Illumina sequencing. Addition of a unique molecular identifier (UMI) sequence enables the accurate bioinformatic identification and removal of PCR duplicates from amplified libraries.
Two sets are available separately:
- C05030010 - D-Plex Single Indexes for Illumina - Set A
- C05030011 - D-Plex Single Indexes for Illumina - Set B
Each set can be used for library multiplexing up to 24. Set A and Set B can be used simultaneously for library multiplexing up to 48.
Read more about the D-Plex technology.
- Publications
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RNA landscapes of brain tissue and brain tissue-derived extracellularvesicles in simian immunodeficiency virus (SIV) infection andSIV-related central nervous system pathology.
Huang Yiyao and Abdelmagid Abdelgawad Ahmed Gamal andTurchinovich Andrey and Queen Suzanne and Abreu CelinaMonteiro and Zhu Xianming and Batish Mona and Zheng Leiand Witwer Kenneth W
Antiretroviral treatment regimens can effectively control HIV replication and some aspects of disease progression. However, molecular events in end-organ diseases such as central nervous system (CNS) disease are not yet fully understood, and routine eradication of latent reservoirs is not yet in reach. Extracellular...Neutral sphingomyelinase 2 inhibition attenuates extracellular vesiclerelease and improves neurobehavioral deficits in murine HIV.
Zhu X. et al.
People living with HIV (PLH) have significantly higher rates of cognitive impairment (CI) and major depressive disorder (MDD) versus the general population. The enzyme neutral sphingomyelinase 2 (nSMase2) is involved in the biogenesis of ceramide and extracellular vesicles (EVs), both of which are dysregulated in PL... - Related products
