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UHRF1 polyclonal antibody
目录号
格式
价格
Polyclonal antibody raised in rabbit against UHRF1 (ubiquitin-like with PHD and ring finger domains 1), using a recombinant protein.
| Lot | 45098 |
|---|---|
| Concentration | 1.4 μg/μl |
| Species reactivity | Human |
| Type | Polyclonal |
| Purity | Affinity purified |
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution * | References |
|---|---|---|
| ChIP assay | 5 μg/ChIP | Fig 1 |
| Immunoprecipitation | 1:100 - 1:500 | Fig 2 |
| Western Blotting | 1:500 - 1:3,000 | Fig 3 |
| Immunohistochemistry | 1:100 - 1:1,000 | Fig 4 |
| Immunofluorescence | 1:500 - 1:1,000 | Fig 5 |
* Optimal dilutions/concentrations should be determined by the researcher.
- Validation Data
Figure 1. UHRF1 ChIP results
ChIP was performed with HCT116 chromatin extract and 5 μg of either control rabbit IgG or UHRF1 antibody. The precipitated DNA was detected by PCR with primer set targeting to PPARG promoter.
Figure 2. IP results
UHRF1 antibody immunoprecipitates UHRF1 protein in IP experiments. IP Sample: HeLa whole cell extract A. 40 μg HeLa whole cell extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of UHRF1 protein by 2 μg of UHRF1 antibody (C15410258) 5% SDS-PAGE The immunoprecipitated UHRF1 protein was detected by western blot with the UHRF1 antibody (C15410258) diluted 1:1,000.
Figure 3. Western blot
Sample: 30 μg of HCT116 whole cell lysate 7.5% SDS PAGE UHRF1 diluted 1:1,000
Figure 4. IHC
UHRF1 antibody detects UHRF1 protein on HBL435 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded HBL435 xenograft. UHRF1 antibody (C15410258) dilution: 1:500.
Figure 5. IFA
Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa cells using UHRF1 antibody (Cat. No. C15410258) (Green) at a 1:500 dilution. Red: Alpha-tubulin filaments. - 出版物
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Novel role of UHRF1 in the epigenetic repression of the latent HIV-1.
Verdikt R. et al.
BACKGROUND: The multiplicity, heterogeneity, and dynamic nature of human immunodeficiency virus type-1 (HIV-1) latency mechanisms are reflected in the current lack of functional cure for HIV-1. Accordingly, all classes of latency-reversing agents (LRAs) have been reported to present variable ex vivo potencies. Here,...DNA methylation enzymes and PRC1 restrict B-cell Epstein-Barr virus oncoprotein expression.
Guo R, Zhang Y, Teng M, Jiang C, Schineller M, Zhao B, Doench JG, O'Reilly RJ, Cesarman E, Giulino-Roth L, Gewurz BE
To accomplish the remarkable task of lifelong infection, the Epstein-Barr virus (EBV) switches between four viral genome latency and lytic programmes to navigate the B-cell compartment and evade immune responses. The transforming programme, consisting of highly immunogenic EBV nuclear antigen (EBNA) and latent membr...