TDG (UniProt/Swiss-Prot entry Q13569) belongs to the TDG/mug DNA glycosylase family. It removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. It is also able to remove thymine from C/T and T/T mispairings, although with lower activity. Furrther, TDG plays a key role in active DNA demethylation as it binds to 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC), but not 5-hydroxymethylcytosine (5hmC), and mediates their excision through base-excision repair (BER) to install an unmethylated cytosine.
TDG monoclonal antibody
目录号
格式
价格
Alternative name: HTDG
Monoclonal antibody raised in mouse against TDG (Thymine-DNA glycosylase), using a recombinant protein.
| Lot | 001 |
|---|---|
| Concentration | 1 μg/μl |
| Species reactivity | Human |
| Type | Monoclonal |
| Purity | Affinity purified |
| Host | Mouse |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| Western Blotting | 1:1,000 | Fig 1 |
| Immunofluorescence | 1:400 | Fig 2 |
- Validation data
Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against TDG
Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against TDG(Cat. No. C15200227) diluted 1:1,000. The position of the TDG and the sumoylated TDG protein is indicated on the right; the marker (in kDa) is shown on the left.
Figure 2. Immunofluorescence using the Diagenode monoclonal antibody directed against TDG
HeLa cells were stained with the Diagenode antibody against TDG (Cat. No. C15200227) diluted 1:400 followed by an anti-mouse antibody conjugated to Alexa488 (left). The right figure shows staining of the nuclei with DAPI. - 出版物
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