SMYD3 (UniProt/Swiss-Prot entry Q9H7B4) is a histone methyltransferase which specifically di- and trimethylates histone H3. It is also able to methylate lysine 5 of histone H4 but does not monomethylate H3K4. SMYD3 is part of an RNA polymerase complex and hence plays an important role in transcriptional activation.
SMYD3 polyclonal antibody
Other names: ZMYND1, ZNFN3A1, KMT3E, BA74P14.1
Polyclonal antibody raised in rabbit against SMYD3 (SET and MYND domain containing 3), using a recombinant protein.
| Lot | 41906 |
|---|---|
| Concentration | 1.32 μg/μl |
| Species reactivity | Human, mouse, rat: positive. Other species: not tested. |
| Type | Polyclonal |
| Purity | Affinity purified polyclonal antibody in PBS containing 20% glycerol and 0.025% ProClin 300. |
| Host | Rabbit |
| Storage Conditions | Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles. |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution * | References |
|---|---|---|
| ChIP* | 5 µg/ChIP | Fig 1 |
| Western blotting | 1:500 - 1:1,000 | Fig 2, 3 |
| Immunoprecipitation | 5 µg/IP | Fig 4 |
| IF | 1:500 | Fig 5 |
* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 µg per IP.
- Validation Data
Figure 1. ChIP results obtained with the Diagenode antibody directed against SMYD3
ChIP was performed on HepG2 cells with 5 µg of the Diagenode antibody against SMYD3 (Cat. No. C15410253). IgG (5 µg/IP) was used as negative IP control. QPCR was performed with primers for the 5’ flanking region of Nkx2.8. Figure 1 shows the fold enrichment over the IgG negative control.
Figure 2. Western blot analysis using the Diagenode antibody directed against SMYD3
Whole cell extracts from 293T cells (30 µg) transfected with a SMYD3 expression vector (lane 2, partial fragment) and untransfected control cells (lane 1) were analysed by western blot using the Diagenode antibody against SMYD3 (Cat. No. C15410253) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
Figure 3. Western blot analysis using the Diagenode antibody directed against SMYD3
Whole cell extracts (30 µg) from 293T, A431, HeLa and HepG2 cells (lane 1, 2, 3 and 4, respectively) were analysed by western blot using the Diagenode antibody against SMYD3 (Cat. No. C15410253) diluted 1:1.000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
Figure 4. Immunoprecipitation using the Diagenode antibody directed against SMYD3
Immunoprecipitation was performed on whole cell extracts from 293T cells using 5 µg of the Diagenode antibody against SMYD3 (Cat. No. C15410253) (lane 2) or with an equal amount of rabbit IgG (lane 1). The immunoprecipitated SMYD3 protein was detected by western blot with the SMYD3 antibody diluted 1:1,000.
Figure 5. Immunofluorescence using the Diagenode antibody directed against SMYD33
HCT116 cells were stained with the Diagenode antibody against SMYD3 (Cat. No. C15410253). Cells were fixed with ice-cold methanol for 5 min and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the SMYD3 antibody (left) diluted 1:500 in blocking solution. The right panel shows costaining with Hoechst 33342. - 出版物
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SMYD3: a new regulator of the early steps of adipocyte differentiation
Gilardi F. et al.
In obesity, adipose tissue undergoes a remodeling process characterized by increased adipocyte size (hypertrophia) and number (hyperplasia). The individual ability to tip the balance toward the hyperplastic growth, with recruitment of new fat cells through adipogenesis, seems to be critical for a healthy adipose tis...