SMAD3S423/425p Polyclonal Antibody

Lot	001
Concentration	1 μg/μl
Species reactivity	Human
Type	Polyclonal
Purity	Affinity purified
Host	Rabbit
Precautions	This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Applications	Suggested dilution	References
Western Blotting	1:2,000	Fig 1
Immunohistochemistry	1:400	Fig 2

Validation data

Figure 1. Western blot analysis using the Diagenode antibody directed against SMAD3S423/425p
Lysates from different cells treated with TGFb (+) or untreated control cells (-) were analysed by Western blot using the Diagenode antibody against SMAD3S423/425p (cat. No. C15410327) diluted 1:2,000. The position of the phosphorylated SMAD3 is indicated on the right. Figure 1 clearly shows the antibody recognizes the phopsphorylated SMAD3 whereas it has no reactivity towards the unphosphorylated SMAD3.

IHC

Figure 2. Immunohistochemistry using the Diagenode antibody directed against SMAD3S423/425p
Formalin fixed paraffin embedded epidermal keratinocytes were stained with the Diagenode antibody against SMAD3S423/425p (cat. No. C15410327) diluted 1:400, followed by a peroxidise labelled anti-rabbit antibody. Figure 2 shows nuclear staining of SMAD3 (red).

Target description

SMAD3 (UniProt/Swiss-Prot entry P84022) belongs to the SMAD (‘mothers against decapentaplegic) family of proteins which are

signal transducers and transcriptional modulators involved in multiple pathways. SMAD3 is activated by TGF-beta (transforming

growth factor) and activin type 1 receptor kinases which phosphorylates the C-terminal serines 423 and 425. SMAD3 binds

the TRE element in the promoter region of many genes that are regulated by TGF-beta and is involved in chondrogenesis,

osteogenesis and wound healing. It is also thought to play a role in carcinogenesis.

应用

WB
Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more

IHC
Immunohistochemistry Read more

文档

SMAD3S423/425p polyclonal antibody DATASHEET Polyclonal antibody raised in rabbit against SMAD3 (SMAD Family Member 3) phosphorylated at Ser42...	Download
Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...	Download
Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...	Download

Safety sheets

SMAD3S423/425p Antibody SDS GB en	Download
SMAD3S423/425p Antibody SDS US en	Download
SMAD3S423/425p Antibody SDS BE nl	Download
SMAD3S423/425p Antibody SDS FR fr	Download
SMAD3S423/425p Antibody SDS BE fr	Download
SMAD3S423/425p Antibody SDS ES es	Download
SMAD3S423/425p Antibody SDS JP ja	Download
SMAD3S423/425p Antibody SDS DE de	Download

出版物

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