NFYA (UniProt/Swiss-Prot entry P23511) is one of the 3 subunits of the ubiquitous transcription factor NFY. All three subunits A, B and C are required to form a NFY-DNA complex. Depending on the cofactors involved, NFY can function both as an activator or a repressor, The NFYA subunit is responsible for the sequence specific interactions of the complex, suggesting a role as regulatory subunit. NFYA regulates the transcription of the core clock component ARNTL/BMAL1.
NFYA Antibody - ChIP-seq Grade
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格式
价格
Alternative names: NF-YA, HAP2, CBF-A, CBF-B
Polyclonal antibody raised in rabbit against NFYA (Nuclear transcription factor-Y, subunit A), using a KLH conjugated synthetic peptide from the N-terminus of the protein.
| Lot | 001 |
|---|---|
| Concentration | not determined |
| Species reactivity | Human |
| Type | Polyclonal ChIP grade, ChIP-seq grade |
| Purity | Whole antiserum |
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ChIP/ChIP-seq * | 1:1,000 | Fig 1, 2 |
| Western Blotting | 1:1,000 | Fig 3 |
| Immunohistochemistry | 1:400 | Fig 4 |
* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μl per IP.
- Validation data
Figure 1. ChIP results obtained with the Diagenode antibody directed against NFYA
ChIP assays were performed using HeLa cells, the Diagenode antibody against NFYA (Cat. No. C15310261) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 μl per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. QPCR was performed with primers for the AURKA and GAPDH promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).A.
B.
C.
D.
Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NFYA
ChIP was performed on sheared chromatin from 4 million HeLa cells using 1 μl of the Diagenode antibody against NFYA (Cat. No. C15310261) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 2 Mb region of the human X chromosome (fig 2A and B), and in a two genomic regions surrounding the AURKA and GAPDH positive control genes.
Figure 3. Western blot analysis using the Diagenode antibody directed against NFYA
Nuclear extracts from CHO-7 cells (35 μg) were analysed by Western blot using the Diagenode antibody against NFYA (Cat. No. C15310261) diluted 1:1,000. Figure 3 shows a doublet representing both isoforms of NFYA.
Figure 4. Immunohistochemistry using the Diagenode antibody directed against NFYA
Formalin fixed paraffin embedded human prostate tissue was stained with the Diagenode antibody against NFYA (cat. No. C15310261) diluted 1:400, followed by a peroxidise labelled anti-rabbit antibody. Figure 2 shows nuclear staining of NFYA (red). - 出版物
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