Human Methylome Service

NEW! Human Methylome enables high coverage genome-wide DNA methylation analysis at the single nucleotide level in Human samples and it is ideal for biomarker discovery. The assay benefits from the advantages of Twist hybrid-capture panel that targets only 83Mb genomic regions containing important DNA methylation site thus enabling a very high CpG coverage. The technology is cost-efficient since it avoids the sequencing of the whole 3.2Gb human genome which includes regions without CpGs. By using Enzymatic Methylation (EM-seq) DNA is converted without further degradation and fragmentation leading to reduced bias and homogenous targeted capture.

High Coverage Genome-wide DNA methylation analysis

• Single nucleotide resolution with enzymatic methylation (EM-seq)
• Compatible with low input and highly fragmented cfDNA and FFPE
• DNA inputs ranging from 200ng down to 10 ng
• Robust (Hybrid-Capture) and cost-effective (reduced sequencing) solution
• High coverage of 200X on CpG containing-region
• Up to 18 million CpGs detected and 9 millions covered more than 10X
• Covers more than 90% of the CpGs included in the Infinium EPIC array
• Suitable for epigenetic biomarker discovery

Complete end-to-end service

• From DNA QC to raw sequencing data
• High-quality bioinformatic support (on request) to assist you with data analysis
• Leveraging
• Applicable to difficult samples such as cfDNA and FFPE
• High CpG average coverage thanks to enzymatic conversion and hybrid-capture