| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
| IP Immunoprecipitation Read more |
| ChIP-qPCR (ab) Read more |
HA tag monoclonal antibody
Monoclonal antibody raised in mouse against the HA tag (amino acid sequence YPYDVPDYA) using a KLH-conjugated synthetic peptide. The HA antibody recognizes the influenza Hemagglutinin epitope which is extensively used as a protein tag in expression vectors. This antibody is extremely specific and allows unambiguous identification and quantitative analysis of HA tagged proteins.
| Lot | 003 |
|---|---|
| Concentration | 1.0 µg/µl |
| Species reactivity | Specific for HA-tagged fusion proteins expressed in bacteria, insect, mammalian cells |
| Type | Monoclonal |
| Purity | Protein A purified |
| Host | Mouse |
| Storage Conditions | Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles. |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution * | References |
|---|---|---|
| Western Blotting | 1:1,000 - 1:3,000 | Fig 1 |
| IP/ChIP | 1:150 - 1:200 | |
| IS | 1:500 - 1:1,000 |
* Please note that optimal antibody amount should be determined by the end-user for each specific assay condition.
- Validation Data
Figure 1. Western blot analysis using the Diagenode monoclonal antibody against the HA protein tag
Western blot was performed on whole cell extracts from HEK293 cells transfected with an HA-tagged expression vector using the Diagenode monoclonal antibody directed against HA (Cat. No. MAb-190-050). The antibody was used at a dilution of 1:1,000. Figure 1 shows the results for untransfected cells, used as a negative control (lane 1) and for cells transfected with a HA-tagged protein (lane 2). The MW marker (in kDa) is shown on the left. A single band is clearly visible in lane 2, but absent in lane 1 showing the high specificity of the HA antibody. - 出版物
How to properly cite our product/service in your work
We strongly recommend using this: HA tag monoclonal antibody (Hologic Diagenode Cat# C15200190 Lot# 003). Click here to copy to clipboard.
Using our products or services in your publication? Let us know!
NUP98 fusion proteins and KMT2A-MENIN antagonize PRC1.1 to drive gene expression in AML
Emily B. Heikamp et al.
Highlights Degradation of NUP98-fp halts nascent transcription of key oncogenes within 1 h NUP98-fp loss results in accumulation of PRC1.1 and repressive histone modifications PRC1.1 is needed for stable gene repression but not for acute transcriptional changes PRC1.1 is requi...A complex epigenome-splicing crosstalk governs epithelial-to-mesenchymaltransition in metastasis and brain development.
Sahu Sanjeeb Kumar et al.
Epithelial-to-mesenchymal transition (EMT) renders epithelial cells migratory properties. While epigenetic and splicing changes have been implicated in EMT, the mechanisms governing their crosstalk remain poorly understood. Here we discovered that a C2H2 zinc finger protein, ZNF827, is strongly induced during variou...Nuclear Receptor Nur77 Limits the Macrophage Inflammatory Response through Transcriptional Reprogramming of Mitochondrial Metabolism.
Koenis DS, Medzikovic L, van Loenen PB, van Weeghel M, Huveneers S, Vos M, Evers-van Gogh IJ, Van den Bossche J, Speijer D, Kim Y, Wessels L, Zelcer N, Zwart W, Kalkhoven E, de Vries CJ
Activation of macrophages by inflammatory stimuli induces reprogramming of mitochondrial metabolism to support the production of pro-inflammatory cytokines and nitric oxide. Hallmarks of this metabolic rewiring are downregulation of α-ketoglutarate formation by isocitrate dehydrogenase (IDH) and accumulation o...FBXO32 promotes microenvironment underlying epithelial-mesenchymal transition via CtBP1 during tumour metastasis and brain development
Sahu S.K. et al.
The set of events that convert adherent epithelial cells into migratory cells are collectively known as epithelial-mesenchymal transition (EMT). EMT is involved during development, for example, in triggering neural crest migration, and in pathogenesis such as metastasis. Here we discover FBXO32, an E3 ubiquitin liga...