ETO Antibody - ChIP-seq Grade

Figure 1. ChIP results obtained with the Diagenode antibody directed against ETO
ChIP assays were performed using SKNO-1 cells, the Diagenode antibody against ETO (Cat. No. C15310001) and optimized primer pairs for qPCR. Sheared chromatin from 1.25 million cells and 4 μl of antibody were used per ChIP experiment. QPCR was performed using primers specific for the FUT7, NFE2, OGG1 and VEGF genes. Figure 1 shows the occupancy, calculated as the ratio + control/background for which the H2B gene was used.

Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against ETO
ChIP was performed as described above. The IP’d DNA of 6 ChIP’s was pooled and analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Figure 2 shows the results of the complete chromosome 3 and three genomic regions surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow.

Figure 3. Determination of the antibody titer
To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human ETO (Cat. No. C15310001). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:1,300.