3-methylcytosine arises by erroneous methylation of DNA through spontaneous exposure to endogenous S-adenosyl methionine (SAM). Since the N3-position in cytosine is protected in double stranded DNA, this phenomenon mainly occurs in single stranded DNA. The resulting 3-mC is toxic and mutagenic and increased levels of 3-mC seem to impair cell proliferation. Therefore, it has to be repaired by the cell. This is established by oxidative demethylation, which is catalyzed by the AlkB protein.
3-methylcytosine (3-mC) polyclonal antibody
目录号
格式
价格
Polyclonal antibody raised in rabbit against 3-methylcytosine (3-mC) conjugated to BSA.
| Lot | A2111-0010 |
|---|---|
| Concentration | 1.80 µg/µl |
| Species reactivity | Human, mouse, other (wide range) |
| Type | Polyclonal |
| Purity | Protein G purified |
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| Dot Blotting | 1:400 | Fig 1 |
* The optimal dilution for other applications should be determined by the end user. For WB we suggest starting with a 1:500 dilution.
- Validation Data
Figure 1. Dot blot analysis using the Diagenode antibody directed against 3-mC
To demonstrate the specificity of the Diagenode antibody against 3-mC (cat. No. C15410209), a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligo’s were spotted on the membrane. The antibody was diluted 1:400 in PBS-T containing 10 % skimmed milk and 1% BSA. Figure 1 shows a high specificity of the antibody for the oligonucleotide with the 3-methylcytosine modification. - 出版物
How to properly cite our product/service in your work
We strongly recommend using this: 3-methylcytosine (3-mC) polyclonal antibody (Hologic Diagenode Cat# C15410209 Lot# A2111-0010). Click here to copy to clipboard.
Using our products or services in your publication? Let us know!
m3C is a mitochondrial mRNA modification which promotes tumor progression
Audrey Penning et al.
Mitochondrial mRNA modifications are suggested to play a role in fine-tuning mitochondrial gene expression and function. However, the epitranscriptomic landscape of mitochondrial mRNA (mt-mRNA) remains poorly explored. Here, we uncover N3-methylcytosine (m3C) as a novel mt-mRNA that is catalyzed by METTL8, an enzyme... - 相关产品
